Medium

Medium(14)=Oat Meal Agar:
Oatmeal (Quaker White Oats) 20.0 g
Trace salts solution (see below) 1.0 g
Agar 18.0 g
Distilled water 1.0 L
Cook or steam 20.0 g of oatmeal in 1.0 L of distilled water for 20 min. Filter through cheesecloth. Make up volume to 1.0 L and add 1.0 ml of trace salt solution. Adjust pH to 7.2 and add 18.0 g agar.
Trace salt solution:
FeSO4.7H2O 0.1 g
MnCl2.4H2O 0.1 g
ZnSO4.7H2O 0.1 g
Distilled water 100.0 ml



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